Wisteria Floribunda Lectin (WFA, WFL), fluorescein (FITC), is a bright-green WFA-fluorophor conjugate. WFA is sourced from Wisteria floribunda (common name: Japanese wisteria), which is a species of flowering plant in the pea family Fabaceae, native to Japan. Due to the specificity of WFA, it has been used in many fields to label certain cells. In neurobiology, it has been used to detect perineuronal net. In the gastrointestinal tract, it stains the foveolar epithelium of normal gastric mucosa. This lectin has also been used as a marker of certain types of ovarian cancer.

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• Very bright FITC conjugate detected with traditional FITC/GFP filter set, Ex/Em: 495/515 nm

• Applications: immunofluorescence, glycobiology

• Sugar specificity: N-acetylgalactosamine

• Inhibiting/eluting sugar: 200 mM N-acetylgalactosamine

• Blood group specify: non-specific

• Unconjugated lectin molecular weight: 116 KDa

• Immunofluorescence working concentration: 5–20 μg/mL

WFA-FITC is supplied at a 2 mg/mL concentration in 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. 0.1 mM Ca2+.

A precipitate may form during storage. This does not have a significant adverse effect on the product. If a precipitate forms upon long-term storage at 4°C, warm to 37°C and centrifuge before use.