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BD Matrigel 胚胎干细胞基质胶, hESC-Qualified Matrix, LDEV-free简介:
产品品牌: BD
产品名称: Matrigel 胚胎干细胞基质胶, hESC-Qualified Matrix, LDEV-free
产品货号: 354277
产品规格: 5mL
保存温度: -20°C
BD Matrigel 胚胎干细胞基质胶, hESC-Qualified Matrix, LDEV-free说明:

Corning Matrigel基质是一种从Engelbreth-Holm Swarm(EHS)小鼠肉瘤中提取的可溶性基底膜制剂,该肿瘤富含细胞外基质蛋白,包括层粘连蛋白(一种主要成分)、IV型胶原、硫酸乙酰肝素蛋白多糖、entactin/nidogen和多种生长因子。
使用要求:
•通过小鼠抗体生产(MAP)测试对小鼠集落进行病原体常规筛查
•进行广泛的PCR检测以筛查多种病原体
•细菌、真菌和支原体检测呈阴性
•Lowry法测定的蛋白质浓度
•通过鲎试剂测定法测定内毒素单位
•基质凝胶基质凝胶稳定性在37°C下测试14天
•使用突起生长测定法测定每个批次的生物活性。
Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.
Quality
-Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
-Extensive PCR testing is performed to screen for a number of pathogens
-Tested and found negative for bacteria, fungi, and mycoplasma
-Protein concentrations determined by Lowry method
-Endotoxin units measured by Limulus Amoebocyte Lysate assay
-Matrigel Matrix gel stability is tested for a period of 14 days at 37°C
-Biological activity is determined for each lot using a neurite outgrowth assay.
Source
Engelbreth-Holm-Swarm (EHS) mouse tumor
Preparation and Storage
Store at -20°C. Avoid multiple freeze-thaws